Composite

Part:BBa_K2169001:Design

Designed by: Filip Cierniak   Group: iGEM16_FAU_Erlangen   (2016-10-14)


ZnO binding Curli protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CsgA is secreted. In order not to interfere with the secretion signal, we had a look at CsgA fusion proteins. C-terminal fusions have been shown to be functional. For cloning into our expression vector with EcoRI, we had to add an RBS before the start codon.


Source

CsgA: E. coli genome/ parts registry

References